Theory for the Liquid–Liquid Phase Separation in Aqueous Antibody Solutions

Phase Separation in Multicomponent Aqueous-Protein Solutions

published in Advance ACS Abstracts, December 1, 1994. 0022-3654/95/2099-0454$09.00/0 we present here the results of experimental and theoretical studies of a ternary solution constituted of the proteins yma (designated as protein A), a “high-Tc” protein, and yw (designated as protein B), a “low-Tc” protein, in aqueous solution. The solution pH was kept constant at 7.1 by a 100 mM phosphate buff...

Monte Carlo study of phase separation in aqueous protein solutions

The binary liquid phase separation of aqueous solutions of g-crystallins is utilized to gain insight into the microscopic interactions between these proteins. The interactions are modeled by a square-well potential with reduced range l and depth e . A comparison is made between the experimentally determined phase diagram and the results of a modified Monte Carlo procedure which combines simulat...

Phase separation during freezing upon warming of aqueous solutions.

Using differential scanning calorimetry, we show that the addition of solute(s) to emulsified water lowers the freezing temperature to <231 K, the homogeneous nucleation temperature of pure bulk water, or even completely suppresses freezing. In the latter case, freezing upon warming occurs above T(X) ≈ 150 K and leads to a phase separation into pure ice and a freeze-concentrated solution (FCS) ...

Theory of solvation-induced reentrant phase separation in polymer solutions.

Separation of methacrylic acid from aqueous phase using quaternary amine

The feasibility of extractive reaction of methacrylic acid from aqueous solution using a quaternary amine, tri–octyl methyl ammonium chloride (TOMAC) as an extractant, was studied. The diluents chosen in the present work belong to different chemical classes, n–butyl acetate, carbon tetrachloride, isoamyl alcohol, methyl isobutyl ketone, and toluene. The effect of initial acid concentration in t...